Quantitative transcript analysis of the inducible expression system pSIP ...
Two sets of overlapping genes, lacLMReu and lacLMAci, encoding heterodimeric beta-galactosidases from Lactobacillus reuteri and Lactobacillus acidophilus, respectively, have previously been cloned and expressed using the pSIP vector systemand Lactobacillus plantarum WCSF1 as host. Despite the high similarity between these lacLM genes and the use of identical cloning and expression strategies, strains harboring lacLMReu produced about twenty-fold more beta-galactosidase than strains containing lacLMAci.Results: In this study, the plasmid copy numbers (PCN) of expression vectors pEH9R (lacLMReu) and pEH9A (lacLMAci) as well as the transcription levels of both lacLM genes were compared using quantitative PCR methods.
Analyses of parallel fermentations of L. plantarum harboring either pEH9R or pEH9A showed that the expression plasmids were present in similar copy numbers.
However, transcript levels of lacLM from L. reuteri (pEH9R) were up to 18 times higher than those of lacLM from L.
acidophilus (pEH9A). As a control, it was shown that the expression levels of regulatory genes involved in pheromone-induced promoter activation were similar in both strains.
Conclusion: The use of identical expression strategies for highly similar genes led to very different mRNA levels.
The data indicate that this difference is primarily caused by translational effects that are likely to affect both mRNA synthesis rates and mRNA stability. These translational effects thus seem to be a dominant determinant for the success of gene expression efforts in lactobacilli.
Inducible Expression System - News
Despite the high similarity between these lacLM genes and the use of identical cloning and expression strategies, strains harboring lacLMReu produced about twenty-fold more beta-galactosidase than strains containing lacLMAci. Results: In this study,
inducible through a comprehensive library of activator ligands; genome engineering featuring stable, location-based integration that doesn't disrupt critical cellular functions; and cell system engineering, which enables controlled expression of
Viral replication leads to Toll-like receptor (TLR) 3, 7, 8, and 9, melanoma differentiation-associated gene 5 (MDA5), retinoic acid-inducible gene I (RIG-I), and protein kinase R (PKR)-dependent production of three types of IFNs that bind to their
iDimerize Inducible Heterodimer System
IGF-I et IGF-II induisent également l'expression de l'hypoxia-inducible factor 1 α (HIF-1α) stimulant en retour une synthèse d'IGF-II ainsi qu'une angiogenèse localement. Il existe une surexpression d'IGF-I, d'IGF-II et d'IGF-IR dans de nombreux types
Induction peptide expression in the nervous system-Yeda Research ...
An effective method for introducing peptides into the brain. Administration of synthetic or purified peptides directly into the brain ventricles is a method commonly used by neuroscientists for exploring physiological and behavioral functions of gene products. Intra-ventricle administration is controlled by the blood-brain barrier, which limits its effectiveness, and currently available methods for the delivery of peptides or pharmacological agents into the cerebrospinal fluid (CSF) in the brain present researchers with limitations, concerning both the natural properties of the delivered substance (solubility, stability, synthesis, purifications, etc) and the experimental needs (prolonged and/or repeated administration, inducible delivery, minimal handling of the experimental animals, etc). The current technology utilizes a genetic approach for the specific delivery of secreted peptides or proteins into the CSF.
The choroid plexus is a blood-CSF barrier, composed of epithelial cells, that plays a critical role in the mechanism regulating the exchange of molecules between the brain's internal milieu and the periphery. Neuromodulators directed to CSF can modify and adapt a variety of behavioral, neuroendocrine, and immunologic processes. The proposed technology is a lentiviral-based system which uses a choroid plexus specific promoter. The functionality of this system was demonstrated by using the over-expression of the two established neuropeptides. This technology may prove as a powerful tool for addressing research questions involving acute or chronic effects of gene products within the CNS.
Press Alert: Quantitative transcript analysis of the inducible expression system pSIP: comparison of the ... 
Quantitative transcript analysis of the inducible expression system pSIP: comparison of the ... - 
Quantitative transcript analysis of the inducible expression system pSIP: comparison of the ... - 
Quantitative transcript analysis of the inducible expression system pSIP: comparison of the ... - 
Quantitative transcript analysis of the inducible expression system pSIP: comparison of the ... - Inducible Expression System - Bookshelf
Molecular biology in medicinal chemistry
The cell-specific expression of CRE recombinase fused to a mutated estrogen ... 2.3.2 The Tetracycline/Doxycycline-Inducible Expression System The ability ...Gene knockout protocols
This natural steroid-inducible system has been manipulated to produce an ecdysone-inducible mammalian expression system that does not appear to affect ...Development of an inducible expression system for Bone Morphogenetic Proteins (BMPs) in mammalian cells
DNA Cloning: Mammalian systems
Controlled expression of foreign genes 75 High level expression 75 Transient expression using T7 RNA polymerase 77 Inducible expression 80 Acknowledgements ...Insect Development, Morphogenesis, Molting and Metamorphosis
Nonsteroidal agonist-inducible expression has been obtained from Zea mays ... attempts to implant an ecdysteroid-inducible gene expression system into cells ...Check Catalog Directory
Tet-On 3G Inducible Expression System
Tet-On 3G is the gold standard tetracycline inducible expression system for mammalian cells, generating the lowest basal expression and highest induction fold.
Lentiviral Tetracycline Inducible Expression Systems
,Lentiviral Tetracycline Inducible Expression Systems ... Clontech's Tet Inducible Systems are sensitive to nanogram concentrations of Doxycycline (a tetracycline ...
RheoSwitch Mammalian Inducible Expression System (E3000 ...
The RheoSwitch<sup></sup> Mammalian Inducible Expression System permits maximum control of gene expression in mammalian cells. Analogous to the operation of a ...
Inducible Protein Expression Using the T-REx™ System
... PCR Products TOPO® Cloning Reaction and Transformation Optimizing the TOPO®Cloning Reaction Transfection Expression and Purification Creation of Stab ...
Complete Control Inducible Mammalian Expression Systems
The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. ...