Assessment of ERBB2 and EGFR gene amplification and protein expression in ...
BackgroundThe goal of this study was to investigate ERBB2HER2 and EGFR gene amplification and protein expression in gastric cancer. Fluorescence in situ hybridization (FISH) and immunohistochemistry were used to analyze ERBB2 and EGFR gene amplification and protein expression in 69 cases of gastric cancer.ResultsFISH analysis revealed that 20.3% of the cases exhibited ERBB2 gene amplification.Increases in ERBB2 copy number and gene amplification were present in 52.1% of the samples. Expression of the ERBB2 protein was observed in 42.0% of cases.
FISH analysis detected EGFR gene amplification in 29.0% of samples. Increases in EGFR copy number and gene amplification occurred in 57.9% of samples, and EGFR protein expression was present in 52.2% of samples.
Both ERBB2 and EGFR gene amplification were 3 cases (4.3%), but abnormalities in both ERBB2 and EGFR gene copy number were present 36.2% of samples. ERBB2 and EGFR gene amplification were significantly associated with the depth of tumor invasion (P 0.05).ConclusionsOur data indicated that ERBB2 and EGFR genetic abnormalities were associated with the prognosis of gastric cancer.
Clinical assessment of ERBB2 and EGFR amplification may represent an important factor for the development of personalized treatment programs for gastic cancer.
In Situ Hybridization - News
Fluorescence in situ hybridization (FISH) and immunohistochemistry were used to analyze ERBB2 and EGFR gene amplification and protein expression in 69 cases of gastric cancer.ResultsFISH analysis revealed that 20.3% of the cases exhibited ERBB2 gene
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The results of immunohistochemical staining together with in situ hybridization for light chains confirmed a mixed lymphocytic population, with CD3 and CD20 positivity and polytypic light-chain expression by plasma cells, and excluded the possibility
[12] Several reproducible detection methods for ALK tyrosine kinase rearrangement have been developed and validated, including break-apart fluorescence in situ hybridization (FISH) to detect chromosomal translocation, multiplexed RT-PCR (reverse
To confirm the findings, Masliza and her husband, Mohd Faiz Abdul Razak, took Iman for a FISH (Fluorescense In Situ Hybridization) examination at a private hospital. The chromosome check confirmed that Iman had Williams Syndrome.
HPV in situ hybridization with catalyzed signal amplification and ...
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We report an unusual case of cerebellar metastasis from a cervical adenosquamous carcinoma in which molecular techniques assisted in establishing the correct diagnosis. The patient was a 43-year-old woman with surgically unresectable cervical carcinoma diagnosed 2 years before presenting with neurological symptoms. A magnetic resonance imaging scan showed a large, enhancing cerebellar lesion with significant brain stem compression. The excised cerebellar tumor resembled a small cell carcinoma and was initially not thought to be a metastasis from the cervical adenosquamous carcinoma.
In Situ Hybridization Kit, Immunohistochemistry Kit and Serv... via 
Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas: Molecular Genetics: Liver and P... 
I am now extracting pCMV-sport 6 plasmid with a Hox1b gene insert so that I can use it for in situ hybridization .... Fun times!
Pcr 3: Pcr in Situ Hybridization : A Practical Approach (The Practical Approach Series): 
In Situ Hybridization Protocols for the Brain (Cloth): In Situ Hybridization - Bookshelf
In situ hybridization, principles and practice
In situ hybridization, a practical approach
In Situ Hybridization, Laboratory Companion
Covering a carefully selected range of techniques with immediate and general applications in research and clinical diagnosis, the book starts with genome and ...In situ hybridization, applications to neurobiology
In situ hybridization, a practical guide
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In situ hybridization - Wikipedia, the free encyclopedia
In situ hybridization of wild type Drosophila embryos at different ... In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary ...
In situ hybridization: Definition from Answers.com
in situ hybridization ( ′in ′sichü ′hībrədəzāshən ) ( cell and molecular biology ) A technique permitting identification of particular
In Situ Hybridization
In the past 30 years many improvements have been made on the basic in situ hybridization technique and its application has been carried out in all different tissues. ...
situ hybridization
The basic principles for in situ hybridization are the same, except one is utilizing the probe to detect specific nucleotide sequences within cells and tissues. ...
In situ hybridization | Abcam
General procedure and tips for in situ hybridization using antibody detection. ... In situ hybridization indicates the localization of gene expression in their cellular ...